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PDIA4 ELISA Kit (Human)

CAT: 0247-OKEH02095-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02095-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Protein disulfide isomerase family A member 4
Gene Aliases
Endoplasmic reticulum resident protein 70; endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; ERP70; ERp-72; ERP72; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) ; protein disulfide isomerase-associated 4; protein disulfide-isomerase A4; testicular tissue protein Li 137.
Gene ID
9601
Accession Number
NP_004902.1
Reactivity
Homo sapiens, Human
Target
Protein disulfide-isomerase A4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PDIA4 ELISA Kit (Human) (OKEH02095) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDIA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDIA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDIA4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
73 kDa
Specificity
Natural and recombinant Human Protein disulfide-isomerase A4
Protein Length
645
NCBI Gene Symbol
PDIA4
Protein Name
P13667
Gene Name URL
PDIA4
Nucleotide Accession Number
NM_004911.4

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