ABCB4 ELISA Kit (Human)

CAT: 0247-OKEH02084-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02084-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
ATP binding cassette subfamily B member 4
Gene Aliases
ABC21; ATP-binding cassette sub-family B member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4; GBD1; ICP3; MDR2; MDR2/3; MDR3; multidrug resistance protein 3; multiple drug resistance 3; PFIC-3; P-glycoprotein 3; P-glycoprotein-3/multiple drug resistance-3; PGY3; phosphatidylcholine translocator ABCB4.
Gene ID
5244
Accession Number
NP_000434.1
Reactivity
Homo sapiens, Human
Target
Phosphatidylcholine translocator ABCB4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ABCB4 ELISA Kit (Human) (OKEH02084) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCB4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCB4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCB4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
34 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
142 kDa
Specificity
Natural and recombinant Human Phosphatidylcholine translocator ABCB4
Protein Length
1286
NCBI Gene Symbol
ABCB4
Protein Name
P21439
Gene Name URL
ABCB4
Nucleotide Accession Number
NM_000443.3

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