PPP1CA ELISA Kit (Human)

CAT: 0247-OKEH01979-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH01979-96WSize:96 Tests
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CAS Number
7732-18-5
Gene Name
Protein phosphatase 1 catalytic subunit alpha
Gene Aliases
PP1A; PP-1A; PP1alpha; PPP1A; protein phosphatase 1, catalytic subunit, alpha isozyme; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; serine/threonine-protein phosphatase PP1-alpha catalytic subunit; testicular tissue protein Li 155.
Gene ID
5499
Accession Number
NP_001008709.1
Reactivity
Homo sapiens, Human
Target
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PPP1CA ELISA Kit (Human) (OKEH01979) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPP1CA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPP1CA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPP1CA captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
0.055 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 kDa
Specificity
Natural and recombinant Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Protein Length
330
NCBI Gene Symbol
PPP1CA
Protein Name
P62136
Gene Name URL
PPP1CA
Nucleotide Accession Number
NM_001008709.1

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