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STS ELISA Kit (Human)

CAT: 0247-OKEH01968-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01968-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Steroid sulfatase
Gene Aliases
ARSC; ARSC1; arylsulfatase C; ASC; ES; estrone sulfatase; SSDD; Steroid sulfatase; steroid sulfatase (microsomal), isozyme S; steryl-sulfatase; steryl-sulfate sulfohydrolase; XLI.
Gene ID
412
Accession Number
NP_000342.2
Reactivity
Homo sapiens, Human
Target
Steryl-sulfatase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology STS ELISA Kit (Human) (OKEH01968) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human STS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated STS and incubated. The STS found in the sample or standards competes with the biotinylated STS for limited binding sites on the immobilized anti-Human STS antibody. Excess unbound biotinylated STS and sample or standard STS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated STS captured in the well and inversely proportional to the amount of STS which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
0.99 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
65 kDa
Specificity
Natural and recombinant Human Steryl-sulfatase
Protein Length
583
NCBI Gene Symbol
STS
Protein Name
P08842
Gene Name URL
STS
Nucleotide Accession Number
NM_000351.4

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