CD63 ELISA Kit (Human)

CAT: 0247-OKEH01943-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH01943-96WSize:96 Tests
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CAS Number
7732-18-5
Gene Name
CD63 molecule
Gene Aliases
CD63 antigen; CD63 antigen (melanoma 1 antigen) ; granulophysin; LAMP-3; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; ocular melanoma-associated antigen; OMA81H; tetraspanin-30; tspan-30; TSPAN30.
Gene ID
967
Accession Number
NP_001244318.1
Reactivity
Homo sapiens, Human
Target
CD63 antigen
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD63 ELISA Kit (Human) (OKEH01943) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD63 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD63 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD63 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
19.5 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Notes
Formerly GWB-TTD47C
Specificity
Natural and recombinant Human CD63 antigen
Protein Length
238
NCBI Gene Symbol
CD63
Protein Name
P08962
Gene Name URL
CD63
Nucleotide Accession Number
NM_001257389.1

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