Products for Research Use Only

ECE1 ELISA Kit (Human)

CAT: 0247-OKEH01929-96WSize: 96 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH01929-96WSize:96 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Gene Name
Endothelin converting enzyme 1
Gene Aliases
ECE; ECE-1; endothelin-converting enzyme 1.
Gene ID
1889
Accession Number
NP_001106818.1
Reactivity
Homo sapiens, Human
Target
Endothelin-converting enzyme 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ECE1 ELISA Kit (Human) (OKEH01929) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ECE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ECE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ECE1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
Sensitivity
20 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
87 kDa
Specificity
Natural and recombinant Human Endothelin-converting enzyme 1
Protein Length
770
NCBI Gene Symbol
ECE1
Protein Name
P42892
Gene Name URL
ECE1
Nucleotide Accession Number
NM_001113347.1