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AVPR1A ELISA Kit (Human)

CAT: 0247-OKEH01915-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01915-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Arginine vasopressin receptor 1A
Gene Aliases
Antidiuretic hormone receptor 1A; AVPR V1a; AVPR1; SCCL vasopressin subtype 1a receptor; V1a vasopressin receptor; V1aR; V1-vascular vasopressin receptor AVPR1A; vascular/hepatic-type arginine vasopressin receptor; vasopressin V1a receptor.
Gene ID
552
Accession Number
NP_000697.1
Reactivity
Homo sapiens, Human
Target
Vasopressin V1a receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AVPR1A ELISA Kit (Human) (OKEH01915) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AVPR1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AVPR1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AVPR1A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.067 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
47 kDa
Specificity
Natural and recombinant Human Vasopressin V1a receptor
Protein Length
418
NCBI Gene Symbol
AVPR1A
Protein Name
P37288
Gene Name URL
AVPR1A
Nucleotide Accession Number
NM_000706.4

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