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INPP5D ELISA Kit (Human)

CAT: 0247-OKEH01875-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01875-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Inositol polyphosphate-5-phosphatase D
Gene Aliases
Hp51CN; Inositol polyphosphate-5-phosphatase of 145 kDa; inositol polyphosphate-5-phosphatase, 145kD; inositol polyphosphate-5-phosphatase, 145kDa; p150Ship; phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; phosphatidylinositol 4,5-bisphosphate 5-phosphatase; SH2 domain-containing inositol 5'-phosphatase 1; SHIP; SHIP1; SHIP-1; signaling inositol polyphosphate 5 phosphatase SIP-145; signaling inositol polyphosphate phosphatase SHIP II; SIP-145.
Gene ID
3635
Accession Number
NP_001017915.1
Reactivity
Homo sapiens, Human
Target
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology INPP5D ELISA Kit (Human) (OKEH01875) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human INPP5D antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated INPP5D and incubated. The INPP5D found in the sample or standards competes with the biotinylated INPP5D for limited binding sites on the immobilized anti-human INPP5D antibody. Excess unbound biotinylated INPP5D and sample or standard INPP5D is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated INPP5D captured in the well and inversely proportional to the amount of INPP5D which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
0.15 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
133 kDa
Specificity
Natural and recombinant Human Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Protein Length
1189
NCBI Gene Symbol
INPP5D
Protein Name
Q92835
Gene Name URL
INPP5D
Nucleotide Accession Number
NM_001017915.2

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