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CYP2C19 ELISA Kit (Human)

CAT: 0247-OKEH01779-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01779-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Cytochrome P450 family 2 subfamily C member 19
Gene Aliases
(R) -limonene 6-monooxygenase; (S) -limonene 6-monooxygenase; (S) -limonene 7-monooxygenase; CPCJ; CYP2C; CYPIIC17; CYPIIC19; cytochrome P450 2C19; cytochrome P-450 II C; cytochrome P450, family 2, subfamily C, polypeptide 19; cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 19; cytochrome P450-11A; cytochrome P450-254C; fenbendazole monooxygenase (4'-hydroxylating) ; flavoprotein-linked monooxygenase; mephenytoin 4'-hydroxylase; mephenytoin 4-hydroxylase; microsomal monooxygenase; P450C2C; P450IIC19; S-mephenytoin 4-hydroxylase; xenobiotic monooxygenase.
Gene ID
1557
Accession Number
NP_000760.1
Reactivity
Homo sapiens, Human
Target
Cytochrome P450 2C19
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CYP2C19 ELISA Kit (Human) (OKEH01779) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP2C19 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP2C19 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP2C19 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.082 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
56 kDa
Specificity
Natural and recombinant Human Cytochrome P450 2C19
Protein Length
490
NCBI Gene Symbol
CYP2C19
Protein Name
P33261
Gene Name URL
CYP2C19
Nucleotide Accession Number
NM_000769.2

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