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PREX2 ELISA Kit (Human)

CAT: 0247-OKEH01730-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01730-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2
Gene Aliases
DEP domain-containing protein 2; DEP.2; DEPDC2; phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein; PPP1R129; P-REX2; protein phosphatase 1, regulatory subunit 129; PtdIns (3,4,5) -dependent Rac exchanger 2.
Gene ID
80243
Accession Number
NP_079146.2
Reactivity
Homo sapiens, Human
Target
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PREX2 ELISA Kit (Human) (OKEH01730) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PREX2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PREX2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PREX2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
Sensitivity
34 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
182 kDa
Specificity
Natural and recombinant Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
Protein Length
1606
NCBI Gene Symbol
PREX2
Protein Name
Q70Z35
Gene Name URL
PREX2
Nucleotide Accession Number
NM_024870.2

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