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TRIM72 ELISA Kit (Mouse)

CAT: 0247-OKEH01716-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01716-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Tripartite motif-containing 72
Gene Aliases
BC067209; MG53; mitsugumin 53; Mitsugumin-53; tripartite motif-containing protein 72.
Gene ID
434246
Accession Number
NP_001073401.1
Reactivity
Mouse, Mus musculus
Target
Tripartite motif-containing protein 72
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TRIM72 ELISA Kit (Mouse) (OKEH01716) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TRIM72 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TRIM72 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TRIM72 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Sensitivity
39.9 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
52 kDa
Specificity
Natural and recombinant Mouse Tripartite motif-containing protein 72
Protein Length
477
NCBI Gene Symbol
Trim72
Protein Name
Q1XH17
Gene Name URL
Trim72
Nucleotide Accession Number
NM_001079932.3

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