RAP1A ELISA Kit (Human)

CAT: 0247-OKEH01696-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH01696-96WSize:96 Tests
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CAS Number
7732-18-5
Gene Name
RAP1A, member of RAS oncogene family
Gene Aliases
C21KG; G-22K; GTP-binding protein smg p21A; KREV1; KREV-1; RAP1; Ras-related protein Krev-1; ras-related protein Rap-1A; SMGP21.
Gene ID
5906
Accession Number
NP_001010935.1
Reactivity
Homo sapiens, Human
Target
Ras-related protein Rap-1A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology RAP1A ELISA Kit (Human) (OKEH01696) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAP1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAP1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAP1A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
Sensitivity
0.056 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Human Ras-related protein Rap-1A
Protein Length
184
NCBI Gene Symbol
RAP1A
Protein Name
P62834
Gene Name URL
RAP1A
Nucleotide Accession Number
NM_001010935.2

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