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PRKCSH ELISA Kit (Human)

CAT: 0247-OKEH01694-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01694-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Protein kinase C substrate 80K-H
Gene Aliases
80K-H protein; advanced glycation end-product receptor 2; AGE-binding receptor 2; AGE-R2; G19P1; GIIB; glucosidase 2 subunit beta; glucosidase II subunit beta; hepatocystin; PCLD; PCLD1; PKCSH; PLD1; protein kinase C substrate 60.1 kDa protein heavy chain; protein kinase C substrate, 80 Kda protein; VASAP-60.
Gene ID
5589
Accession Number
NP_001001329.1
Reactivity
Homo sapiens, Human
Target
Glucosidase 2 subunit beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRKCSH ELISA Kit (Human) (OKEH01694) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRKCSH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRKCSH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRKCSH captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.24 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
59 kDa
Specificity
Natural and recombinant Human Glucosidase 2 subunit beta
Protein Length
528
NCBI Gene Symbol
PRKCSH
Protein Name
P14314
Gene Name URL
PRKCSH
Nucleotide Accession Number
NM_001001329.2

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