PEG10 ELISA Kit (Human)

CAT: 0247-OKEH01691-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH01691-96WSize:96 Tests
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CAS Number
7732-18-5
Gene Name
Paternally expressed 10
Gene Aliases
EDR; embryonal carcinoma differentiation regulated; Embryonal carcinoma differentiation-regulated protein; HB-1; mammalian retrotransposon-derived 2; mammalian retrotransposon-derived protein 2; Mar2; Mart2; MEF3 like 1; MEF3L; myelin expression factor 3-like protein 1; paternally expressed gene 10 protein; retrotransposon gag domain-containing protein 3; retrotransposon Gag like 2; retrotransposon-derived gag-like polyprotein; retrotransposon-derived protein PEG10; RGAG3; RTL2; SIRH1; Sushi-Ichi retrotransposon homolog 1; ty3/Gypsy-like protein.
Gene ID
23089
Accession Number
NP_001035242.1
Reactivity
Homo sapiens, Human
Target
Retrotransposon-derived protein PEG10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PEG10 ELISA Kit (Human) (OKEH01691) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PEG10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PEG10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PEG10 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.19 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
80 kDa
Specificity
Natural and recombinant Human Retrotransposon-derived protein PEG10
Protein Length
708
NCBI Gene Symbol
PEG10
Protein Name
Q86TG7
Gene Name URL
PEG10
Nucleotide Accession Number
NM_001040152.1

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