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IFIH1 ELISA Kit (Human)

CAT: 0247-OKEH01672-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01672-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Interferon induced with helicase C domain 1
Gene Aliases
AGS7; CADM-140 autoantigen; clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; helicard; helicase with 2 CARD domains; Hlcd; IDDM19; interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA5; MDA-5; melanoma differentiation-associated gene 5; melanoma differentiation-associated protein 5; murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116; SGMRT1.
Gene ID
64135
Accession Number
NP_071451.2
Reactivity
Homo sapiens, Human
Target
Interferon-induced helicase C domain-containing protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IFIH1 ELISA Kit (Human) (OKEH01672) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFIH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFIH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFIH1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
117 kDa
Specificity
Natural and recombinant Human Interferon-induced helicase C domain-containing protein 1
Protein Length
1025
NCBI Gene Symbol
IFIH1
Protein Name
Q9BYX4
Gene Name URL
IFIH1
Nucleotide Accession Number
NM_022168.3

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