FERMT2 ELISA Kit (Mouse)

CAT: 0247-OKEH01664-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01664-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Fermitin family member 2
Gene Aliases
AA960555; fermitin family homolog 2; Kind; Kindlin-2; Mig; Mig2; mitogen inducible 2; pleckstrin homology domain containing, family C (with FERM domain) member 1; pleckstrin homology domain-containing family C member 1; Plekh; Plekhc1.
Gene ID
218952
Accession Number
NP_666166.2
Reactivity
Mouse, Mus musculus
Target
Fermitin family homolog 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FERMT2 ELISA Kit (Mouse) (OKEH01664) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FERMT2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FERMT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FERMT2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Sensitivity
39.2 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
77 kDa
Specificity
Natural and recombinant Mouse Fermitin family homolog 2
Protein Length
680
NCBI Gene Symbol
Fermt2
Protein Name
Q8CIB5
Gene Name URL
Fermt2
Nucleotide Accession Number
NM_146054.2

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