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CD5L ELISA Kit (Mouse)

CAT: 0247-OKEH01656-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01656-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
CD5 antigen-like
Gene Aliases
1/6; A; AAC-; AAC-11; AI047839; AIM; AIM/Spalpha; Api6; apoptosis inhibitor 6; apoptosis inhibitor expressed by macrophages; Apoptosis inhibitory 6; CD5 antigen-like; CT2; CT-2; mAIM; Pdp; Pdp 1/6; Sp; Sp-alpha.
Gene ID
11801
Accession Number
NP_033820.2
Reactivity
Mouse, Mus musculus
Target
CD5 antigen-like
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD5L ELISA Kit (Mouse) (OKEH01656) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD5L has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD5L is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD5L captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
15.63 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
38 kDa
Specificity
Natural and recombinant Mouse CD5 antigen-like
Protein Length
352
NCBI Gene Symbol
Cd5l
Protein Name
Q9QWK4
Gene Name URL
Cd5l
Nucleotide Accession Number
NM_009690.2

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