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B4GALT5 ELISA Kit (Human)

CAT: 0247-OKEH01652-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01652-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Beta-1,4-galactosyltransferase 5
Gene Aliases
B4Gal-T5; beta-1,4-galactosyltransferase 5; beta-1,4-GalT II; beta-1,4-GalT IV; beta-1,4-GalTase 5; beta-1.4-galactosyltransferase V; beta4-GalT IV; beta4Gal-T5; BETA4-GALT-IV; beta4GalT-V; glucosylceramide beta-1,4-galactosyltransferase; gt-V; lacCer synthase; lactosylceramide synthase; UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5.
Gene ID
9334
Accession Number
NP_004767.1
Reactivity
Homo sapiens, Human
Target
Beta-1,4-galactosyltransferase 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology B4GALT5 ELISA Kit (Human) (OKEH01652) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human B4GALT5 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated B4GALT5 and incubated. The B4GALT5 found in the sample or standards competes with the biotinylated B4GALT5 for limited binding sites on the immobilized anti-human B4GALT5 antibody. Excess unbound biotinylated B4GALT5 and sample or standard B4GALT5 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated B4GALT5 captured in the well and inversely proportional to the amount of B4GALT5 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.067 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
45 kDa
Specificity
Natural and recombinant Human Beta-1,4-galactosyltransferase 5
Protein Length
388
NCBI Gene Symbol
B4GALT5
Protein Name
O43286
Gene Name URL
B4GALT5
Nucleotide Accession Number
NM_004776.3