KL ELISA Kit (Mouse)

CAT: 0247-OKEH01597-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01597-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Klotho
Gene Aliases
Alp; alpha-kl; klotho; secreted form of Klotho protein.
Gene ID
16591
Accession Number
NP_038851.2
Reactivity
Mouse, Mus musculus
Target
Klotho
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology KL ELISA Kit (Mouse) (OKEH01597) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KL captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
7.92 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
116 kDa
Notes
Formerly GWB-OFSD8Q
Specificity
Natural and recombinant Mouse Klotho
Protein Length
1014
NCBI Gene Symbol
Kl
Protein Name
O35082
Gene Name URL
Kl
Nucleotide Accession Number
NM_013823.2

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