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BMX ELISA Kit (Human)

CAT: 0247-OKEH01576-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01576-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
BMX non-receptor tyrosine kinase
Gene Aliases
Bone marrow tyrosine kinase gene in chromosome X protein; BTK-like on X chromosome; cytoplasmic tyrosine-protein kinase BMX; epithelial and endothelial tyrosine kinase; ETK; Etk/Bmx cytosolic tyrosine kinase; NTK38; NTK38 tyrosine kinase; PSCTK2; PSCTK3.
Gene ID
660
Accession Number
NP_001712.1
Reactivity
Homo sapiens, Human
Target
Cytoplasmic tyrosine-protein kinase BMX
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BMX ELISA Kit (Human) (OKEH01576) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMX captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
78 kDa
Specificity
Natural and recombinant Human Cytoplasmic tyrosine-protein kinase BMX
Protein Length
675
NCBI Gene Symbol
BMX
Protein Name
P51813
Gene Name URL
BMX
Nucleotide Accession Number
NM_001721.6

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