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LPCAT3 ELISA Kit (Human)

CAT: 0247-OKEH01563-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01563-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Lysophosphatidylcholine acyltransferase 3
Gene Aliases
1-acylglycerophosphocholine O-acyltransferase;1-acylglycerophosphoethanolamine O-acyltransferase;1-acylglycerophosphoserine O-acyltransferase; C3F; LPCAT; LPLAT 5; LPSAT; lyso-PC acyltransferase 3; Lysophosphatidylcholine acyltransferase; Lysophosphatidylcholine acyltransferase 3; lysophosphatidylserine acyltransferase; lysophospholipid acyltransferase 5; lyso-PS acyltransferase; MBOAT5; membrane bound O-acyltransferase domain containing 5; membrane-bound O-acyltransferase domain-containing protein 5; nessy; OACT5; O-acyltransferase (membrane bound) domain containing 5; O-acyltransferase domain-containing protein 5; putative protein similar to nessy.
Gene ID
10162
Accession Number
NP_005759.4
Reactivity
Homo sapiens, Human
Target
Lysophospholipid acyltransferase 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LPCAT3 ELISA Kit (Human) (OKEH01563) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LPCAT3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LPCAT3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LPCAT3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
56 kDa
Specificity
Natural and recombinant Human Lysophospholipid acyltransferase 5
Protein Length
487
NCBI Gene Symbol
LPCAT3
Protein Name
Q6P1A2
Gene Name URL
LPCAT3
Nucleotide Accession Number
NM_005768.5

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