CD24 ELISA Kit (Human)

CAT: 0247-OKEH01549-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01549-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
CD24 molecule
Gene Aliases
CD24 antigen (small cell lung carcinoma cluster 4 antigen) ; CD24A; signal transducer CD24; Small cell lung carcinoma cluster 4 antigen.
Gene ID
100133941
Accession Number
NP_001278666.1
Reactivity
Homo sapiens, Human
Target
Signal transducer CD24
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD24 ELISA Kit (Human) (OKEH01549) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD24 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD24 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD24 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
8 kDa
Specificity
Natural and recombinant Human Signal transducer CD24
Protein Length
80
NCBI Gene Symbol
CD24
Protein Name
P25063
Gene Name URL
CD24
Nucleotide Accession Number
NM_001291737.1

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