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PRKACB ELISA Kit (Human)

CAT: 0247-OKEH01413-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01413-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Protein kinase cAMP-activated catalytic subunit beta
Gene Aliases
CAFD2; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PKACB; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, beta.
Gene ID
5567
Accession Number
NP_001229786.1
Reactivity
Homo sapiens, Human
Target
CAMP-dependent protein kinase catalytic subunit beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRKACB ELISA Kit (Human) (OKEH01413) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRKACB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRKACB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRKACB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
20 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
41 kDa
Specificity
Natural and recombinant Human cAMP-dependent protein kinase catalytic subunit beta
Protein Length
351
NCBI Gene Symbol
PRKACB
Protein Name
P22694
Gene Name URL
PRKACB
Nucleotide Accession Number
NM_001242857.2

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