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TNC ELISA Kit (Human)

CAT: 0247-OKEH01405-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01405-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Tenascin C
Gene Aliases
150-225; cytotactin; deafness, autosomal dominant 56; DFNA56; glioma-associated-extracellular matrix antigen; GMEM; GP; GP 150-225; Hexabrachion; hexabrachion (tenascin) ; HXB; JI; myotendinous antigen; neuronectin; tenascin; Tenascin-C; tenascin-C additional domain 1; TN; TN-C.
Gene ID
3371
Accession Number
NP_002151.2
Reactivity
Homo sapiens, Human
Target
Tenascin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TNC ELISA Kit (Human) (OKEH01405) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TNC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TNC and incubated. The TNC found in the sample or standards competes with the biotinylated TNC for limited binding sites on the immobilized anti-human TNC antibody. Excess unbound biotinylated TNC and sample or standard TNC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TNC captured in the well and inversely proportional to the amount of TNC which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
20 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
241 kDa
Specificity
Natural and recombinant Human Tenascin
Protein Length
2201
NCBI Gene Symbol
TNC
Protein Name
P24821
Gene Name URL
TNC
Nucleotide Accession Number
NM_002160.3

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