CDKN2C ELISA Kit (Human)

• Gene Name:

  Cyclin dependent kinase inhibitor 2C

• Gene Aliases:

  CDK6 inhibitor p18; cyclin-dependent inhibitor; cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; cyclin-dependent kinase 6 inhibitor p18; cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4) ; INK4C; p18; p18-INK4C; p18-INK6.

• Gene ID:

  1031

• Accession Number:

  NP_001253.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Cyclin-dependent kinase 4 inhibitor C

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology CDKN2C ELISA Kit (Human) (OKEH01381) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDKN2C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDKN2C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDKN2C captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

• Detection Range:

  0.156-10ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%

• Sensitivity:

  0.1 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  18 kDa

• Specificity:

  Natural and recombinant Human Cyclin-dependent kinase 4 inhibitor C

• Protein Length:

  168

• NCBI Gene Symbol:

  CDKN2C

• Protein Name:

  P42773

• Gene Name URL:

  CDKN2C

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001262.2