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ROMO1 ELISA Kit (Mouse)

CAT: 0247-OKEH01372-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01372-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Reactive oxygen species modulator 1
Gene Aliases
2010100O12Rik; AI853864; protein MGR2 homolog; reactive oxygen species modulator 1; ROS modulator 1.
Gene ID
67067
Accession Number
NP_001157688.1
Reactivity
Mouse, Mus musculus
Target
Reactive oxygen species modulator 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ROMO1 ELISA Kit (Mouse) (OKEH01372) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ROMO1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ROMO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ROMO1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
Sensitivity
8.06 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
8 kDa
Specificity
Natural and recombinant Mouse Reactive oxygen species modulator 1
Protein Length
79
NCBI Gene Symbol
Romo1
Protein Name
P60603
Gene Name URL
Romo1
Nucleotide Accession Number
NM_001164216.1

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