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MASP1 ELISA Kit (Human)

CAT: 0247-OKEH01355-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01355-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
MBL associated serine protease 1
Gene Aliases
3MC1; C4/C2 activating component of Ra-reactive factor; complement factor MASP-3; complement-activating component of Ra-reactive factor; CRARF; CRARF1; mannan binding lectin serine peptidase 1; mannan-binding lectin serine protease 1; mannose-associated serine protease 1; mannose-binding lectin-associated serine protease 1; mannose-binding protein-associated serine protease; MAP1; MAP-1; MAp44; MASP; MASP3; MASP-3; PRSS5; Ra-reactive factor serine protease p100; RaRF; serine protease 5.
Gene ID
5648
Accession Number
NP_001027019.1
Reactivity
Homo sapiens, Human
Target
Mannan-binding lectin serine protease 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MASP1 ELISA Kit (Human) (OKEH01355) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MASP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MASP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MASP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
28 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
79 kDa
Specificity
Natural and recombinant Human Mannan-binding lectin serine protease 1
Protein Length
699
NCBI Gene Symbol
MASP1
Protein Name
P48740
Gene Name URL
MASP1
Nucleotide Accession Number
NM_001031849.2

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