EIF3A ELISA Kit (Human)

CAT: 0247-OKEH01347-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01347-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Eukaryotic translation initiation factor 3 subunit A
Gene Aliases
Centrosomin homolog; cytoplasmic protein p167; EIF3; eIF3 p167; eIF3 p180; eIF3 p185; EIF3, p180 subunit; eIF3-p170; EIF3S10; eIF3-theta; eIF-3-theta; eukaryotic translation initiation factor 3 subunit 10; eukaryotic translation initiation factor 3 subunit A; eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD) ; eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD) ; eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10, 170kD; P167; p180; p185; TIF32.
Gene ID
8661
Accession Number
NP_003741.1
Reactivity
Homo sapiens, Human
Target
Eukaryotic translation initiation factor 3 subunit A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EIF3A ELISA Kit (Human) (OKEH01347) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EIF3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EIF3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EIF3A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.23 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
167 kDa
Specificity
Natural and recombinant Human Eukaryotic translation initiation factor 3 subunit A
Protein Length
1382
NCBI Gene Symbol
EIF3A
Protein Name
Q14152
Gene Name URL
EIF3A
Nucleotide Accession Number
NM_003750.2

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