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NFKB1 ELISA Kit (Mouse)

CAT: 0247-OKEH01301-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01301-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
Gene Aliases
DNA-binding factor KBF1; EBP-1; NF kappaB1; NF-k; NF-kappaB; NF-kappaB p50; NF-kappaB1; NF-kappa-B1 p84/NF-kappa-B1 p98; NF-KB1; nuclear factor kappaB p50; nuclear factor NF-kappa-B p105 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105; p5; p50; p50 subunit of NF kappaB; p50/p1; p50/p105.
Gene ID
18033
Accession Number
NP_032715.2
Reactivity
Mouse, Mus musculus
Target
Nuclear factor NF-kappa-B p105 subunit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NFKB1 ELISA Kit (Mouse) (OKEH01301) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFKB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NFKB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFKB1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.091 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
106 kDa
Specificity
Natural and recombinant Mouse Nuclear factor NF-kappa-B p105 subunit
Protein Length
971
NCBI Gene Symbol
Nfkb1
Protein Name
P25799
Gene Name URL
Nfkb1
Nucleotide Accession Number
NM_008689.2

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