NFKB1 ELISA Kit (Mouse)

• Gene Name:

  Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105

• Gene Aliases:

  DNA-binding factor KBF1; EBP-1; NF kappaB1; NF-k; NF-kappaB; NF-kappaB p50; NF-kappaB1; NF-kappa-B1 p84/NF-kappa-B1 p98; NF-KB1; nuclear factor kappaB p50; nuclear factor NF-kappa-B p105 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105; p5; p50; p50 subunit of NF kappaB; p50/p1; p50/p105.

• Gene ID:

  18033

• Accession Number:

  NP_032715.2

• Reactivity:

  Mouse, Mus musculus

• Target:

  Nuclear factor NF-kappa-B p105 subunit

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology NFKB1 ELISA Kit (Mouse) (OKEH01301) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFKB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NFKB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFKB1 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

• Detection Range:

  0.156-10ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

• Sensitivity:

  0.091 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  106 kDa

• Specificity:

  Natural and recombinant Mouse Nuclear factor NF-kappa-B p105 subunit

• Protein Length:

  971

• NCBI Gene Symbol:

  Nfkb1

• Protein Name:

  P25799

• Gene Name URL:

  Nfkb1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_008689.2