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VCAN ELISA Kit (Human)

CAT: 0247-OKEH01296-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01296-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Versican
Gene Aliases
Chondroitin sulfate proteoglycan core protein 2; CSPG2; ERVR; GHAP; glial hyaluronate-binding protein; large fibroblast proteoglycan; PG-M; versican core protein; versican proteoglycan; WGN; WGN1.
Gene ID
1462
Accession Number
NP_001119808.1
Reactivity
Homo sapiens, Human
Target
Versican core protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology VCAN ELISA Kit (Human) (OKEH01296) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VCAN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VCAN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VCAN captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
373 kDa
Specificity
Natural and recombinant Human Versican core protein
Protein Length
3396
NCBI Gene Symbol
VCAN
Protein Name
P13611
Gene Name URL
VCAN
Nucleotide Accession Number
NM_001126336.2

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