TMSB4X ELISA Kit (Mouse)

• Gene Name:

  Thymosin, beta 4, X chromosome

• Gene Aliases:

  Prothymosin beta 4; Ptmb; Ptmb4; t beta 4; Tb4; Tbeta; Tbeta4; thymosin beta-4.

• Gene ID:

  19241

• Accession Number:

  NP_067253.1

• Reactivity:

  Mouse, Mus musculus

• Target:

  Thymosin beta-4

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology TMSB4X ELISA Kit (Mouse) (OKEH01277) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse TMSB4X antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TMSB4X and incubated. The TMSB4X found in the sample or standards competes with the biotinylated TMSB4X for limited binding sites on the immobilized anti-mouse TMSB4X antibody. Excess unbound biotinylated TMSB4X and sample or standard TMSB4X is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TMSB4X captured in the well and inversely proportional to the amount of TMSB4X which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

• Detection Range:

  0.312-20ng/ml

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

• Sensitivity:

  0.168 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  6 kDa

• Specificity:

  Natural and recombinant Mouse Thymosin beta-4

• Protein Length:

  50

• NCBI Gene Symbol:

  Tmsb4x

• Protein Name:

  P20065

• Gene Name URL:

  Tmsb4x

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_021278.2