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TMSB4X ELISA Kit (Mouse)

CAT: 0247-OKEH01277-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01277-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Thymosin, beta 4, X chromosome
Gene Aliases
Prothymosin beta 4; Ptmb; Ptmb4; t beta 4; Tb4; Tbeta; Tbeta4; thymosin beta-4.
Gene ID
19241
Accession Number
NP_067253.1
Reactivity
Mouse, Mus musculus
Target
Thymosin beta-4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology TMSB4X ELISA Kit (Mouse) (OKEH01277) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse TMSB4X antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TMSB4X and incubated. The TMSB4X found in the sample or standards competes with the biotinylated TMSB4X for limited binding sites on the immobilized anti-mouse TMSB4X antibody. Excess unbound biotinylated TMSB4X and sample or standard TMSB4X is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TMSB4X captured in the well and inversely proportional to the amount of TMSB4X which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.168 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
6 kDa
Specificity
Natural and recombinant Mouse Thymosin beta-4
Protein Length
50
NCBI Gene Symbol
Tmsb4x
Protein Name
P20065
Gene Name URL
Tmsb4x
Nucleotide Accession Number
NM_021278.2

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