OIT3 ELISA Kit (Human)

CAT: 0247-OKEH01240-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01240-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Oncoprotein induced transcript 3
Gene Aliases
Liver-specific zona pellucida domain-containing protein; liver-specific ZP domain-containing protein; LZP; oncoprotein-induced transcript 3 protein.
Gene ID
170392
Accession Number
NP_689848.1
Reactivity
Homo sapiens, Human
Target
Oncoprotein-induced transcript 3 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology OIT3 ELISA Kit (Human) (OKEH01240) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OIT3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OIT3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OIT3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
0.076 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
60 kDa
Specificity
Natural and recombinant Human Oncoprotein-induced transcript 3 protein
Protein Length
545
NCBI Gene Symbol
OIT3
Protein Name
Q8WWZ8
Gene Name URL
OIT3
Nucleotide Accession Number
NM_152635.2

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