SFTPC ELISA Kit (Human)

CAT: 0247-OKEH01168-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01168-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Surfactant protein C
Gene Aliases
BRICD6; BRICHOS domain containing 6; PSP-C; pulmonary surfactant apoprotein-2 SP-C; pulmonary surfactant-associated protein C; pulmonary surfactant-associated proteolipid SPL (Val) ; SFTP2; SMDP2; SP5; SP-C.
Gene ID
6440
Accession Number
NP_001165828.1
Reactivity
Homo sapiens, Human
Target
Pulmonary surfactant-associated protein C
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SFTPC ELISA Kit (Human) (OKEH01168) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SFTPC captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.469-30ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Human Pulmonary surfactant-associated protein C
Protein Length
197
NCBI Gene Symbol
SFTPC
Protein Name
P11686
Gene Name URL
SFTPC
Nucleotide Accession Number
NM_001172357.1

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