NARG2 ELISA Kit (Human)

CAT: 0247-OKEH01118-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01118-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Interactor of little elongation complex ELL subunit 2
Gene Aliases
BRCC1; breast cancer cell 1; interactor of little elongator complex ELL subunit 2; little elongation complex subunit 2; NARG2; NMDA receptor regulated 2; NMDA receptor-regulated gene 2; NMDA receptor-regulated protein 2.
Gene ID
79664
Accession Number
NP_001018099.1
Reactivity
Homo sapiens, Human
Target
Little elongation complex subunit 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NARG2 ELISA Kit (Human) (OKEH01118) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NARG2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NARG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NARG2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
110 kDa
Specificity
Natural and recombinant Human NMDA receptor-regulated protein 2
Protein Length
982
NCBI Gene Symbol
ICE2
Protein Name
Q659A1
Gene Name URL
ICE2
Nucleotide Accession Number
NM_001018089.2

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