Products for Research Use Only
RegisterLogin

AMY1A ELISA Kit (Human)

CAT: 0247-OKEH01050-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH01050-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Gene Name
Amylase alpha 1A, amylase alpha 1B, amylase alpha 1C
Gene Aliases
1,4-alpha-D-glucan glucanohydrolase 1; alpha-amylase 1; alpha-amylase 1A; alpha-amylase 1B; alpha-amylase 1C; AMY1; amylase alpha 1A (salivary) ; amylase alpha 1B (salivary) ; amylase alpha 1C (salivary) ; amylase, salivary, alpha-1A; amylase, salivary, alpha-1B; amylase, salivary, alpha-1C; glycogenase; salivary alpha-amylase; salivary amylase alpha 1A; salivary amylase alpha 1B; salivary amylase alpha 1C.
Gene ID
276, 277, 278
Accession Number
NP_001008219.1
Reactivity
Homo sapiens, Human
Target
Alpha-amylase 1, Alpha-amylase 1A, Alpha-amylase 1B, Alpha-amylase 1C
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AMY1A ELISA Kit (Human) (OKEH01050) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human AMY1A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AMY1A and incubated. The AMY1A found in the sample or standards competes with the biotinylated AMY1A for limited binding sites on the immobilized anti-human AMY1A antibody. Excess unbound biotinylated AMY1A and sample or standard AMY1A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AMY1A captured in the well and inversely proportional to the amount of AMY1A which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
Sensitivity
1.56 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
58 kDa
Specificity
Natural and recombinant Human Alpha-amylase 1
Protein Length
511
NCBI Gene Symbol
AMY1A, AMY1B, AMY1C
Protein Name
P04745
Gene Name URL
AMY1A, AMY1B, AMY1C
Nucleotide Accession Number
NM_001008218.1

Related Products

CatalogName
OACA06813-50ULAMY1A Antibody
OACA06813-100ULAMY1A Antibody
OCOA16648-20UGAMY1A Protein Lysate
OCOA13327-20UGAMY1A Protein Lysate
ARP54700_P050-25ULAmy1a Antibody - middle region
ARP97696_P050AMY1A Antibody Middle region
OPCD01281-10UGAMY1A more Recombinant Protein
ARP97696_P050-25ULAMY1A Antibody - middle region
OPCD01281-50UGAMY1A more Recombinant Protein
OPCD01281-200UGAMY1A more Recombinant Protein

Alternative Products

CatalogName
ASB-OKCD07429-96WAMY1A ELISA Kit (Human)
ELA-E1482hHuman AMY1A ELISA Kit
KBH20130GENLISA™ Human Salivary Alpha Amylase (AMY1A) ELISA
ELI-04866hHuman Alpha-amylase 1, AMY1A ELISA KIT
EKU02323Human Salivary Alpha Amylase (AMY1A) ELISA Kit
MBS9921798-01Human Amylase, Alpha 1A (AMY1A) ELISA Kit
EH24785Human AMY1A (Salivary Alpha Amylase) ELISA Kit
E0140HuHuman AMY1A/Alpha-amylase 1 ELISA Kit
abx575213-01Human Amylase Alpha 1, Salivary (AMY1A) ELISA Kit
abx378499-01Human Alpha-Amylase 1A (AMY1A) ELISA Development Kit

Popular Products