Products for Research Use Only

EFNA1 ELISA Kit (Human)

CAT: 0247-OKEH01001-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH01001-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Gene Name
Ephrin A1
Gene Aliases
B61; ECKLG; EFL1; eph-related receptor tyrosine kinase ligand 1; ephrin-A1; epididymis secretory sperm binding protein; EPLG1; gastric cancer metastasis associated long noncoding RNA; GMAN; immediate early response protein B61; LERK1; LERK-1; ligand of eph-related kinase 1; TNF alpha-induced protein 4; TNFAIP4; Tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4.
Gene ID
1942
Accession Number
NP_004419.2
Reactivity
Homo sapiens, Human
Target
Ephrin-A1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EFNA1 ELISA Kit (Human) (OKEH01001) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EFNA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EFNA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EFNA1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.098 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
24 kDa
Specificity
Natural and recombinant Human Ephrin-A1
Protein Length
205
NCBI Gene Symbol
EFNA1
Protein Name
P20827
Gene Name URL
EFNA1
Nucleotide Accession Number
NM_004428.2