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SOCS2 ELISA Kit (Human)

CAT: 0247-OKEH00997-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00997-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Suppressor of cytokine signaling 2
Gene Aliases
CIS2; CIS-2; Cish2; cytokine-inducible SH2 protein 2; SOCS-2; SSI2; SSI-2; STATI2; STAT-induced STAT inhibitor 2; suppressor of cytokine signaling 2.
Gene ID
8835
Accession Number
NP_001257396.1
Reactivity
Homo sapiens, Human
Target
Suppressor of cytokine signaling 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SOCS2 ELISA Kit (Human) (OKEH00997) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SOCS2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SOCS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SOCS2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
125-8000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
Sensitivity
56 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
22 kDa
Specificity
Natural and recombinant Human Suppressor of cytokine signaling 2
Protein Length
198
NCBI Gene Symbol
SOCS2
Protein Name
O14508
Gene Name URL
SOCS2
Nucleotide Accession Number
NM_001270467.1

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