FTMT ELISA Kit (Human)

CAT: 0247-OKEH00934-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00934-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Ferritin mitochondrial
Gene Aliases
Ferritin H subunit; ferritin, mitochondrial; mitochondrial ferritin; MTF.
Gene ID
94033
Accession Number
NP_803431.1
Reactivity
Homo sapiens, Human
Target
Ferritin, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FTMT ELISA Kit (Human) (OKEH00934) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FTMT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FTMT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FTMT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.32 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Human Ferritin, mitochondrial
Protein Length
242
NCBI Gene Symbol
FTMT
Protein Name
Q8N4E7
Gene Name URL
FTMT
Nucleotide Accession Number
NM_177478.1

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