FTMT ELISA Kit (Human)
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FTMT ELISA Kit (Human)
Gene Name:
Ferritin mitochondrialGene Aliases:
Ferritin H subunit; ferritin, mitochondrial; mitochondrial ferritin; MTF.Gene ID:
94033Accession Number:
NP_803431.1Reactivity:
Homo sapiens, HumanTarget:
Ferritin, mitochondrialType:
ELISA KitApplications:
Enzyme-linked Immunosorbent assay-SandwichAssay Principle:
Aviva Systems Biology FTMT ELISA Kit (Human) (OKEH00934) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FTMT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FTMT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FTMT captured in well.Assay Protocol:
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) ProtocolSample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluidsDetection Range:
0.625-40ng/mLRecovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%Sensitivity:
0.32 ng/mLReconstitution:
Store as indicated in product manual.Molecular Weight:
28 kDaSpecificity:
Natural and recombinant Human Ferritin, mitochondrialProtein Length:
242NCBI Gene Symbol:
FTMTProtein Name:
Q8N4E7Gene Name URL:
FTMTCAS Number:
7732-18-5Nucleotide Accession Number:
NM_177478.1
