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CRH ELISA Kit (Mouse)

CAT: 0247-OKEH00624-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00624-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Corticotropin releasing hormone
Gene Aliases
Corticoliberin; corticotropin releasing factor; Corticotropin-releasing factor; Corticotropin-releasing hormone; CR; CRF; Gm1347.
Gene ID
12918
Accession Number
NP_991338.1
Reactivity
Mouse, Mus musculus
Target
Corticoliberin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Crh ELISA Kit (Mouse) (OKEH00624) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog Crh antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Crh and incubated. The Crh found in the sample or standards competes with the biotinylated Crh for limited binding sites on the immobilized anti-dog Crh antibody. Excess unbound biotinylated Crh and sample or standard Crh is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Crh captured in the well and inversely proportional to the amount of Crh which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
20 kDa
Specificity
Natural and recombinant Mouse Corticoliberin
Protein Length
187
NCBI Gene Symbol
Crh
Protein Name
Q8CIT0
Gene Name URL
Crh
Nucleotide Accession Number
NM_205769.3

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