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HSP90B1 ELISA Kit (Human)

CAT: 0247-OKEH00613-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH00613-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Heat shock protein 90 beta family member 1
Gene Aliases
94 kDa glucose-regulated protein; ECGP; endoplasmin; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; GP96; gp96 homolog; GRP94; heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; HEL35; HEL-S-125m; stress-inducible tumor rejection antigen gp96; TRA1; tumor rejection antigen (gp96) 1; tumor rejection antigen 1.
Gene ID
7184
Accession Number
NP_003290.1
Reactivity
Homo sapiens, Human
Target
Endoplasmin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HSP90B1 ELISA Kit (Human) (OKEH00613) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSP90B1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSP90B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSP90B1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.041 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
92 kDa
Specificity
Natural and recombinant Human Endoplasmin
Protein Length
803
NCBI Gene Symbol
HSP90B1
Protein Name
P14625
Gene Name URL
HSP90B1
Nucleotide Accession Number
NM_003299.2