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CCK ELISA Kit (Human)

CAT: 0247-OKEH00596-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00596-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Cholecystokinin
Gene Aliases
Cholecystokinin; cholecystokinin triacontatriapeptide; prepro-cholecystokinin.
Gene ID
885
Accession Number
NP_000720.1
Reactivity
Homo sapiens, Human
Target
Cholecystokinin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology CCK ELISA Kit (Human) (OKEH00596) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CCK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CCK and incubated. The CCK found in the sample or standards competes with the biotinylated CCK for limited binding sites on the immobilized anti-human CCK antibody. Excess unbound biotinylated CCK and sample or standard CCK is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CCK captured in the well and inversely proportional to the amount of CCK which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
Natural and recombinant Human Cholecystokinin
Protein Length
115
NCBI Gene Symbol
CCK
Protein Name
P06307
Gene Name URL
CCK
Nucleotide Accession Number
NM_000729.5

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