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GRINA ELISA Kit (Rat)

CAT: 0247-OKEH00576-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00576-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Glutamate ionotropic receptor NMDA type subunit associated protein 1
Gene Aliases
Glutamate [NMDA] receptor-associated protein 1; glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding) ; NMDA receptor glutamate-binding chain; NMDA receptor glutamate-binding subunit; N-methyl-D-aspartate receptor glutamate-binding chain; protein lifeguard 1.
Gene ID
266668
Accession Number
NP_695220.4
Reactivity
Rat, Rattus norvegicus
Target
Protein lifeguard 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GRINA ELISA Kit (Rat) (OKEH00576) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat GRINA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Rat GRINA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat GRINA captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Sensitivity
40.2 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 kDa
Specificity
Natural and recombinant Rat Protein lifeguard 1
Protein Length
348
NCBI Gene Symbol
Grina
Protein Name
Q6P6R0
Gene Name URL
Grina
Nucleotide Accession Number
NM_153308.4

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