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MAPK3 ELISA Kit (Rat)

CAT: 0247-OKEH00560-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00560-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Mitogen activated protein kinase 3
Gene Aliases
Erk-1; ERK1; ERT2; Esrk1; extracellular signal-regulated kinase 1; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK1; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; MNK1; p44; p44erk1; p44-ERK1; p44mapk; Prkm3.
Gene ID
50689
Accession Number
NP_059043.1
Reactivity
Rat, Rattus norvegicus
Target
Mitogen-activated protein kinase 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAPK3 ELISA Kit (Rat) (OKEH00560) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat MAPK3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Rat MAPK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat MAPK3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 kDa
Specificity
Natural and recombinant Rat Mitogen-activated protein kinase 3
Protein Length
380
NCBI Gene Symbol
Mapk3
Protein Name
P21708
Gene Name URL
Mapk3
Nucleotide Accession Number
NM_017347.2

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