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PLAT ELISA Kit (Human)

CAT: 0247-OKEH00496-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00496-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Plasminogen activator, tissue type
Gene Aliases
Alteplase; plasminogen/activator kringle; reteplase; tissue-type plasminogen activator; TPA; T-PA; t-plasminogen activator.
Gene ID
5327
Accession Number
NP_000921.1
Reactivity
Homo sapiens, Human
Target
Tissue-type plasminogen activator
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PLAT ELISA Kit (Human) (OKEH00496) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human PLAT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human PLAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human PLAT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.056 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
63 kDa
Specificity
Natural and recombinant Human Tissue-type plasminogen activator
Protein Length
562
NCBI Gene Symbol
PLAT
Protein Name
P00750
Gene Name URL
PLAT
Nucleotide Accession Number
NM_000930.4

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