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UBQLN2 ELISA Kit (Human)

CAT: 0247-OKEH00484-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00484-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Ubiquilin 2
Gene Aliases
ALS15; CHAP1; DSK2; DSK2 homolog; HRIHFB2157; N4BP4; Nedd4 binding protein 4; PLIC2; protein linking IAP with cytoskeleton 2; ubiquilin-2; ubiquitin-like product Chap1/Dsk2.
Gene ID
29978
Accession Number
NP_038472.2
Reactivity
Homo sapiens, Human
Target
Ubiquilin-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology UBQLN2 ELISA Kit (Human) (OKEH00484) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human UBQLN2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human UBQLN2 and incubated. The Human UBQLN2 found in the sample or standards competes with the biotinylated Human UBQLN2 for limited binding sites on the immobilized anti-Human UBQLN2 antibody. Excess unbound biotinylated Human UBQLN2 and sample or standard Human UBQLN2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human UBQLN2 captured in the well and inversely proportional to the amount of Human UBQLN2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
34 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
66 kDa
Specificity
Natural and recombinant Human Ubiquilin-2
Protein Length
624
NCBI Gene Symbol
UBQLN2
Protein Name
Q9UHD9
Gene Name URL
UBQLN2
Nucleotide Accession Number
NM_013444.3

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