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VIP ELISA Kit (Human)

CAT: 0247-OKEH00406-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00406-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Vasoactive intestinal peptide
Gene Aliases
PHM27; prepro-VIP; VIP peptides.
Gene ID
7432
Accession Number
NP_003372.1
Reactivity
Homo sapiens, Human
Target
VIP peptides
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology VIP ELISA Kit (Human) (OKEH00406) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human VIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human VIP and incubated. The Human VIP found in the sample or standards competes with the biotinylated Human VIP for limited binding sites on the immobilized anti-Human VIP antibody. Excess unbound biotinylated Human VIP and sample or standard human VIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human VIP captured in the well and inversely proportional to the amount of human VIP which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100pg/mL
Sensitivity
0.78 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Specificity
Natural and recombinant Human VIP peptides
Protein Length
170
NCBI Gene Symbol
VIP
Protein Name
P01282
Gene Name URL
VIP
Nucleotide Accession Number
NM_003381.3

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