APOH ELISA Kit (Human)

7732-18-5

Apolipoprotein H

Activated protein C-binding protein; anticardiolipin cofactor; APC inhibitor; apo-H; Apolipoprotein H; apolipoprotein H (beta-2-glycoprotein I) ; B2G1; B2GP1; B2GPI; beta (2) GPI; beta-2-glycoprotein 1; Beta-2-glycoprotein I; BG; epididymis secretory sperm binding protein.

350

NP_000033.2

Homo sapiens, Human

Beta-2-glycoprotein 1

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology APOH ELISA Kit (Human) (OKEH00375) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for APOH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APOH captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

0.78-50ng/ml

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%

0.33 ng/mL

Store as indicated in product manual.

38 kDa

Natural and recombinant Human Beta-2-glycoprotein 1

345

APOH

P02749

APOH