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TIE1 ELISA Kit (Mouse)

CAT: 0247-OKEH00213-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00213-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Gene Aliases
D430008P04Rik; TI; TIE; tie-; tie-1; tyrosine kinase receptor 1; tyrosine-protein kinase receptor Tie-1.
Gene ID
21846
Accession Number
NP_035717.2
Reactivity
Mouse, Mus musculus
Target
Tyrosine-protein kinase receptor Tie-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TIE1 ELISA Kit (Mouse) (OKEH00213) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TIE1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for TIE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TIE1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
125 kDa
Specificity
Natural and recombinant Mouse Tyrosine-protein kinase receptor Tie-1
Protein Length
1134
NCBI Gene Symbol
Tie1
Protein Name
Q06806
Gene Name URL
Tie1
Nucleotide Accession Number
NM_011587.2

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