TNFRSF8 ELISA Kit (Mouse)

CAT: 0247-OKEH00034-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00034-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Tumor necrosis factor receptor superfamily, member 8
Gene Aliases
Cd30; CD30L receptor; D1S166E; Ki; Ki-1; lymphocyte activation antigen CD30; tumor necrosis factor receptor superfamily member 8.
Gene ID
21941
Accession Number
NP_033427
Reactivity
Mouse, Mus musculus
Target
Tumor necrosis factor receptor superfamily member 8
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TNFRSF8 ELISA Kit (Mouse) (OKEH00034) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFRSF8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFRSF8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFRSF8 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
7.89 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
53 kDa
Specificity
Natural and recombinant Mouse Tumor necrosis factor receptor superfamily member 8
Protein Length
498
NCBI Gene Symbol
Tnfrsf8
Protein Name
Q60846
Gene Name URL
Tnfrsf8
Nucleotide Accession Number
NM_009401

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