BDNF ELISA Kit (Mouse)

CAT: 0247-OKEH00022-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00022-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
CAS Number
7732-18-5
Gene Name
Brain derived neurotrophic factor
Gene Aliases
Brain-derived neurotrophic factor.
Gene ID
12064
Accession Number
NP_001041604
Reactivity
Mouse, Mus musculus
Target
Brain-derived neurotrophic factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BDNF ELISA Kit (Mouse) (OKEH00022) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BDNF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BDNF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
0.08 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Mouse Brain-derived neurotrophic factor
Protein Length
249
NCBI Gene Symbol
Bdnf
Protein Name
P21237
Gene Name URL
Bdnf
Nucleotide Accession Number
NM_001048139

Related Products

CatalogName
OKEH00021-96WBDNF ELISA Kit (Human)
OKEH00023-96WBDNF ELISA Kit (Rat)
OKCA01604-96WBDNF ELISA Kit (Monkey)
OKBB00676-96WBDNF ELISA Kit (Rabbit)
OKCD05621-96WBDNF ELISA Kit (Human)
OKBB00127-96WBDNF ELISA Kit (Human)
OKEH03798-96WBDNF ELISA Kit (Dog)
OKBB00675-96WBDNF ELISA Kit (Monkey)
OKCD05627-96WBDNF ELISA Kit (Rabbit)
OKEH07221-96WBDNF ELISA Kit (Bovine)

Alternative Products

CatalogName
KT-1553Mouse BDNF ELISA
MBS9135966-01Mouse BDNF ELISA Kit
MBS355435-01BDNF Mouse ELISA Kit
MBS495345-01BDNF (Mouse) ELISA Kit
143726BDNF BioAssayTM ELISA Kit, Mouse
EK0309Mouse BDNF ELISA Kit PicoKine®
KA0331Mouse BDNF ELISA Kit (Colorimetric)
MBS175800-01Mouse BDNF PicoKine ELISA Kit
LF-EK50014Mouse BDNF ELISA kit (4X96T)
GR117020Nori® Mouse BDNF ELISA Kit

Popular Products